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The effect of cholesterol on the solubility limits of different SMs in the Ld phase. The solubility of the SMs in the Ld phase was measured at 23°C in POPC (A) and PAPC (B) bilayers containing 0 or 20 mol % cholesterol. Above these concentrations, the SMs formed ordered domains. The values are averages of ≥3 experiments ± SD.
