Table 3.
Relative expression of muscarinic receptor subtypes (M1 to M3) in mouse peripheral airways
| M1 | M2 | M3 | |
|---|---|---|---|
| Brain | 20.19 (1.0) | 22.94 (1.0) | 22.27 (1.0) |
| Airways 1 | 34.61 (0.000) | 29.00 (0.029) | 30.18 (0.008) |
| Airways 2 | Undetectable | 29.93 (0.018) | 30.52 (0.007) |
| Airways 3 | 34.36 (0.000) | 30.38 (0.015) | 28.78 (0.028) |
| Airways 4 | 34.68 (0.000) | 29.85 (0.019) | 28.80 (0.025) |
| Airways 5 | 34.93 (0.000) | 29.83 (0.016) | 28.66 (0.022) |
| Average (1–5) | (0.000) | (0.019) | (0.018) |
| SE | (0.000) | (0.003) | (0.004) |
Quantitative RT-PCR data shown are CT values followed by the fold difference (in parentheses) in gene expression (2−ΔΔCT) obtained with the ΔΔCT method using GAPDH as the reference gene and brain tissue as the calibrator sample. Each sample contained ~15 peripheral airways microdissected from several precision-cut lung slices (PCLS) from a different mouse (n = 5 mice) as described in materials and methods.