FIGURE 3.

Effect of furosemide on pendrin abundance and distribution in the cortex and medulla. Top panel shows renal cortical sections labeled for pendrin from mice that received 7 days of a NaCl-replete diet or diet and furosemide. As shown, in both the cortical collecting duct (CCD) (C and D) and connecting tubule (CNT) (A and B), cells that label for pendrin are much larger with more pendrin label in each cell in sections taken from the furosemide-treated mice. [From Pech et al. (140).] Bottom panel shows pendrin label in sections from the same mice at lower magnification. As shown, pendrin label is much more prominent in the cortex of furosemide-treated than of vehicle-treated mice. In the medulla, few pendrin-positive cells are observed in mice from either group.