Figure 3.

Applications of DNA Base Editors

(A) DNA base editors can be used for versatile therapeutic tools for disease treatment or disease modeling. (B) CBEs can be used for converting a CAA, CAG, CGA, or TGG codon into a premature stop codon (TAA, TAG, or TGA), which abolishes protein synthesis and results in a knockout of gene function. (C) ABE can be used for converting the adenine in a start codon (ATG) into a guanine to abolish protein synthesis. (D) ABE can be used for converting a premature stop codon into a codon for an amino acid. Although the resulting codon is not always the same as the unmutated original, whole protein synthesis is no longer abolished. (E) CBEs can be used for converting Gs within splicing acceptor sites into As by editing Cs in the complementary strand of the target site. (F) BEs can be used for generating various substitutions for screening experiments.