Figure 8.

Regulatory T Cell Levels Are Increased in the Lungs and dLNs of Allograft-Recipient Mice following TGF-β MSC Administration

(A–C) Total (A) CD3⁺, (B) CD3⁺CD4⁺, and (C) CD3⁺CD8⁺ T cell frequencies in the lungs, spleens, and dLNs of untreated and TGF-β MSC-treated corneal allograft-recipient mice were investigated by flow cytometry at POD 19. (D and E) Frequency (%) of CD3⁺CD4⁺CD44^loCD62L⁻CD25⁺ (D) and CD3⁺CD8⁺CD44^loCD62L⁻CD25⁺ (E) activated effector T cells in the lungs, spleens, and dLNs of untreated and TGF-β MSC-treated corneal allograft-recipient mice at POD 19 are shown. (F) Flow cytometry gating strategy used to select CD4⁺Foxp3⁺ Tregs. (G) Frequency (%) of CD4⁺Foxp3⁺ Tregs in the lungs, spleens, and dLNs of untreated and TGF-β MSC-treated corneal allograft-recipient mice at POD 19. Error bars show mean ± SD. ∗p < 0.05, ∗∗p < 0.01. A D’Agostino and Pearson omnibus normality test and Shapiro-Wilk normality test were used to determine distribution of data. ROUT testing was used to identify outliers. A parametric unpaired two-tailed Student’s t test was used for data that were normally distributed. A non-parametric unpaired two-tailed Student’s t test was used for data that were not normally distributed (each individual dot represents a separate animal; n = 4–8).