Figure 5.

Accumulation of CYCA3 Proteins throughout the Cell Cycle.

(A) to (O) GUS signal at 7 DAS in root tips of CYCA3;1_pro:CYCA3;1-GUS ([A] to [E]), CYCA3;2_pro:CYCA3;2-GUS ([F] to [J]), and CYCA3;4_pro:CYCA3;4-GUS ([K] to [O]) synchronized by treating with 2 mM hydroxyurea for the indicated periods. The 0-h time point represents untreated control conditions ([A], [F], and [K]), while 6 h corresponds with S phase ([B], [G], and [L]), 10 h with G₂ ([C], [H], and [M]), 17 h with G₂/M ([D], [I], and [N]), and 20 h with M/G₁ ([E], [J], and [O]). Per line and time point, six images were taken. Scale bars represent 50 µm.