Figure 5.

MG53 attenuated myocardial oxidative stress in SIMD rats, which was abolished by GW6471. Oxidative stress injury was detected through (a) representative DHE stained images depicting in situ superoxide production (red, 400x magnification, scale bar = 50 μm), (b) quantification of DHE-signal intensity (folds of the sham group), (c) superoxide dismutase (SOD), (d) malondialdehyde (MDA), (e) glutathione (GSH) activity, and (f) free 15-F2t-isoprostane levels in the myocardium via an ELISA. Data are presented as mean ± SD, n = 8. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001. CLP = cecal ligation and puncture; rhMG53 = recombinant human mitsugumin-53.