Figure 7.

The protein expression of TGF-β1 and α-SMA in HK-2 cells detected by western blotting. (a) The protein expression of TGF-β1 and α-SMA in HK-2 cells of each group. The expression of TGF-β1/GAPDH (b) and α-SMA/GAPDH (c) was quantitatively analyzed in each group. NG: normal glucose (5.5 mM); HG: high glucose (60 mM); EGCG: HK-2 cells treated with 1 μM EGCG; Klotho KO: Klotho gene knockout; Klotho OE: Klotho gene overexpression. Values are expressed as means ± SEM. ^∗∗P < 0.01 vs. the HG group.