Figure 1.

Schematics of the S segment of wildtype lymphocytic choriomeningitis virus (wtLCMV) and various mutated recombinant LCMVs (rLCMVs). The number above the region name (5′ untranslated region (UTR), intergenic region (IGR), and 3′ UTR) indicates the number of the nucleic acid in these regions. Red colored regions and the ordinal numbers described below indicate the deleted regions of S segment. Green colored regions and the ordinal numbers described below indicate the region which have complementary nucleic sequences of the relevant ordinal numbers. Blue colored regions and the ordinal numbers indicate the regions in which nucleic acid sequence of 5′ and 3′ termini are replaced by each other. All ordinal numbers are counted from either 5′ or 3′ termini, whichever is the nearest. The S segment structures of each virus names described on the left side of the schematics correspond to each schematic shown in the right side. The details on how each LCMVs were generated are described in the Plasmids subsection and in the Transfection, Minigenome, and Reverse Genetics System subsection in the Methods section.