Table 1.

The 5′ nucleotide sequences of untranslated regions (UTRs) of plasmids for the reverse genetics and minigenome systems.

Plasmid	5′ UTR Nucleotide Sequence (from 5′ to 3′, vRNA Polarity)
pRF-WE-SRG or SMG	CGCACCGGGGATCCTAGGCTTTTTGGATTGCGCTTTCCTTTAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG (or SMG)-5UTRΔ20–40	CGCACCGGGGATCCTAGGC---------------------TAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG (or SMG)-5UTRΔ41–60	CGCACCGGGGATCCTAGGCTTTTTGGATTGCGCTTTCCTT--------------------TTCTATCCAGTAAAAGG
pRF-WE-SRG (or SMG) -5UTRΔ60–77	CGCACCGGGGATCCTAGGCTTTTTGGATTGCGCTTTCCTTTAGGACAACTGGGTGCTGG------------------
pRF-WE-SRG (or SMG)-3UTRΔ20–38	CGCACCGGGGATCCTAGGCTTTTTGGATTGCGCTTTCCTTTAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG (or SMG)-3UTRΔ39–60	CGCACCGGGGATCCTAGGCTTTTTGGATTGCGCTTTCCTTTAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG (or SMG)-UTR-comple	CGCACCGGGGATCCTAGGCCCAAAAAATTGCGCTTTCCTTTAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG-UTR 5-3 change	CGCACCGGGGATCCTAGGCTAAA--CTAACGCGAAAATAATAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG-Δ26–40	CGCACCGGGGATCCTAGGCTTTTTG---------------TAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG-Δ20–25	CGCACCGGGGATCCTAGGC------GATTGCGCTTTCCTTTAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG-Δ20–30	CGCACCGGGGATCCTAGGC-----------CGCTTTCCTTTAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG
pRF-WE-SRG-Δ31–40	CGCACCGGGGATCCTAGGCTTTTTGGATTG----------TAGGACAACTGGGTGCTGGATTCTATCCAGTAAAAGG

Underscores indicate mutated nucleotide sequences. Hyphens indicate deletion of nucleotide sequences in these positions.