Table 2.

The 3′ nucleotide sequences of UTRs of plasmids for the reverse genetics and minigenome systems.

Plasmid	3′ UTR Nucleotide Sequence (from 3′ to 5′, cRNA Polarity)
pRF-WE-SRG or SMG	GCGTGTCACCTAGGATCCGTAAA--CTAACGCGAAAATAAACCTTTAAGTAACACACTGTTT
pRF-WE-SRG (or SMG)-5UTRΔ20–40	GCGTGTCACCTAGGATCCGTAAA--CTAACGCGAAAATAAACCTTTAAGTAACACACTGTTT
pRF-WE-SRG (or SMG)-5UTRΔ41–60	GCGTGTCACCTAGGATCCGTAAA--CTAACGCGAAAATAAACCTTTAAGTAACACACTGTTT
pRF-WE-SRG (or SMG)-5UTRΔ60–77	GCGTGTCACCTAGGATCCGTAAA--CTAACGCGAAAATAAACCTTTAAGTAACACACTGTTT
pRF-WE-SRG (or SMG)-3UTRΔ20–38	GCGTGTCACCTAGGATCCG---------------------ACCTTTAAGTAACACACTGTTT
pRF-WE-SRG (or SMG)-3UTRΔ39–60	GCGTGTCACCTAGGATCCGTAAA--CTAACGCGAAAATAA----------------------
pRF-WE-SRG (or SMG)-UTR-comple	GCGTGTCACCTAGGATCCG-GTTTA-TAACGCGAAAATAAACCTTTAAGTAACACACTGTTT
pRF-WE-SRG-UTR 5-3 change	GCGTGTCACCTAGGATCCGTTTTTGGATTGCGCTTTCCTTACCTTTAAGTAACACACTGTTT
pRF-WE-SRG-Δ26–40	GCGTGTCACCTAGGATCCGTAAA-----------------ACCTTTAAGTAACACACTGTTT
pRF-WE-SRG-Δ20–25	GCGTGTCACCTAGGATCCG------CTAACGCGAAAATAAACCTTTAAGTAACACACTGTTT
pRF-WE-SRG-Δ20–30	GCGTGTCACCTAGGATCCG-----------GCGAAAATAAACCTTTAAGTAACACACTGTTT
pRF-WE-SRG-Δ31–40	GCGTGTCACCTAGGATCCGTAAA-CTAAC-----------ACCTTTAAGTAACACACTGTTT

Underscores indicate mutated nucleotide sequences. Hyphens indicate deletion of nucleotide sequences in these positions.