Table 3.
RNA secondary structure prediction of the LCMV S segment RNA produced from each plasmid.
| Plasmid Name | Number of Nucleotides Forming a Panhandle Structure at the Termini | Base Pairs with High Base-Pairing Probability, Except for the 19 Base Pairs at the Termini | ||
|---|---|---|---|---|
| At the 5′ Terminus | At the 3′ Terminus | At the 5′ Terminus | At the 3′ Terminus | |
| pRF-WE-SRG | 45 nt | 42 nt | 28–33 | 26–31 |
| pRF-WE-SRG-5UTRΔ20–40 | 19 nt | 19 nt | none | none |
| pRF-WE-SRG-5UTRΔ41–60 | 36 nt | 34 nt | 28–33 | 26–31 |
| pRF-WE-SRG-5UTRΔ60–77 | 45 nt | 42 nt | 28–33 | 26–31 |
| pRF-WE-SRG-3UTRΔ20–38 | 27 nt | 23 nt | none | none |
| pRF-WE-SRG-3UTRΔ39–60 | 36 nt | 34 nt | 28–33 | 26–31 |
| pRF-WE-SRG-UTR-comple | 45 nt | 42 nt | 28–33 | 26–31 |
| pRF-WE-SRG-UTR 5-3 change | 34 nt | 36 nt | 26–31 | 28–33 |
| pRF-WE-SRG-Δ26–40 | 25 nt | 25 nt | none | none |
| pRF-WE-SRG-Δ20–25 | 39 nt | 38 nt | 20–27 | 20–27 |
| pRF-WE-SRG-Δ20–30 | 34 nt | 33 nt | 20–22 | 20–22 |
| pRF-WE-SRG-Δ31–40 | 35 nt | 32 nt | none | none |