Table 4.
Viral growth efficiency in vitro and pathogenicity in vivo for recombinant LCMVs generated from each plasmid.
| Plasmid Name | Virus Name | Viral Growth Efficiency In Vitro Compared to That of rtLCMV in Vero Cell | Viral Pathogenicity In Vivo (Mortality Rate) | ||
|---|---|---|---|---|---|
| CBA/NSlc Mice | DBA/1JJmsSlc Mice | Immunity Acquired | |||
| pRF-WE-SRG | rwtLCMV # | 80% | 60% | Acquired | |
| pRF-WE-SRG-5UTRΔ20–40 | rLCMV-5UTRΔ20–40 | None | Not done (ND) | ND | ND |
| pRF-WE-SRG-5UTRΔ41–60 | rLCMV-5UTRΔ41–60 | Equal | 40% | 0% | Acquired |
| pRF-WE-SRG-5UTRΔ60–77 | rLCMV-5UTRΔ60–77 | Equal | 80% | 0% | Acquired |
| pRF-WE-SRG-3UTRΔ20–38 | rLCMV-3UTRΔ20–38 | None | ND | ND | ND |
| pRF-WE-SRG-3UTRΔ39–60 | rLCMV-3UTRΔ39–60 ## | Less | 0% | 20% | Acquired |
| pRF-WE-SRG-UTR-comple | rLCMV-UTR-comple | Less | 0% | 0% | Acquired * |
| pRF-WE-SRG-UTR 5-3 change | rLCMV-UTR 5-3 change | Less | 0% | 0% | Acquired ** |
| pRF-WE-SRG-Δ26–40 | rLCMV-Δ26–40 | Less | 0% | 0% | Acquired *** |
| pRF-WE-SRG-Δ20–25 | rLCMV-Δ20–25 | None | ND | ND | ND |
| pRF-WE-SRG-Δ20–30 | rLCMV-Δ20–30 | None | ND | ND | ND |
| pRF-WE-SRG-Δ31–40 | rLCMV-Δ31–40 | None | ND | ND | ND |
# Viral growth efficiency of rwtLCMV in Vero cells is equal that of wtLCMV. ## Viral growth efficiency of rLCMV-3UTRΔ39–60 in Vero cells is equal that of wtLCMV. * One DBA/1JJmsSlc mouse infected with rLCMV-UTR-comple showed ruffled fur and weight loss. ** All DBA/1JJmsSlc mice showed ruffled fur and weight loss. *** All DBA/1JJmsSlc mice showed ruffled fur and weight loss, and one of five mice died.