Table 5.
Mutations acquired through the serial passage in Vero cells.
| Virus Name | Segment | Nt Position (vRNA Sense) |
P0 * | P10 ** | Gene Name | Amino Acid Change | Remarks |
|---|---|---|---|---|---|---|---|
| rLCMV-5UTRΔ60–77 | L | 929 | C | T and C *** | L | V2091I | |
| rLCMV-UTR-comple | S | 20 | C | C and T *** | 5’ UTR | The authentic nucleic acid sequence of wtLCMV at the 20th position was T. | |
| S | 21 | C | C and T *** | 5’ UTR | The authentic nucleic acid sequence of wtLCMV at the 21st position was T. | ||
| S | 3355 | T | T and A *** | 3’ UTR | The authentic nucleic acid sequence of wtLCMV at the 3355th position was A. | ||
| S | 3356 | G | G and A *** | 3’ UTR | The authentic nucleic acid sequence of wtLCMV at the 3356th position was A. | ||
| rLCMV-UTR 5-3 change | S | 32 | A | C and A *** | 5’ UTR | ||
| S | 969 | T | T and C *** | GPC | C298C | Synonymous change. | |
| S | 3334 | C | T and C *** | 3’ UTR | |||
| L | 2463 | A | G and A *** | L | C1579C | Synonymous change. | |
| L | 2524 | A | G and A *** | L | V1559A | ||
| L | 2614 | A | G and A *** | L | L1529P | ||
| L | 2689 | A | G and A *** | L | F1504S | ||
| L | 3757 | C | T and C *** | L | S1148N | ||
| L | 6698 | A | G and A *** | L | L168L | Synonymous change. | |
| L | 6699 | A | G and A *** | L | L167L | Synonymous change. | |
| rLCMV-Δ26–40 | L | 2708 | C | T | L | E1498K | |
| L | 5332 | T | A | L | K623M |
* P0 indicates the nucleic acid sequence of original rLCMVs. ** P10 indicates the nucleic acid sequence of serially passaged rLCMVs. *** Sequences represented by “x” and “y” in the P10 column indicate that the nucleic acid sequence of this nt position was a mixture of “x” and “y” and the major nucleic acid was “x”.