Fig. 6.

Overexpression of Ad-G6PD₅₄₅-green fluorescent protein (GFP) increased KCl-induced bovine coronary artery (BCA) contraction. A and B: BCAs were transfected with adenoviral vector (Ad) alone (Ad-Ctrl-GFP; n = 6); Ad-G6PD₅₄₅-GFP (n = 8); and Ad-G6PD₅₁₅-GFP (n = 5). Glucose-6-phosphate dehydrogenase (G6PD) expression was determined by Western blot analyses using anti-GFP antibody and its activity was measured in the BCA homogenates. GAPDH was used as loading control. Unt, untransfected. C: the graph summarizes the peak contractions evoked by 2 concentrations of KCl (30 mmol/L, 30 K and 120 mmol/L, 120 K) in BCAs overexpressing empty Ad-Ctrl-GFP (n = 9 in 30 KCl and 120 KCl each), Ad-G6PD₅₄₅-GFP (n = 5 in 30 KCl and 6 in 120 KCl), and Ad-G6PD₅₁₅-GFP (n = 5 in 30 KCl and 120 KCl each), respectively. *P < 0.05 vs. Ad-Ctrl-GFP.