Fig. 7.

Glucose-6-phosphate dehydrogenase (G6PD) inhibition and deficiency decreased KCl-induced bovine coronary artery (BCA) contraction. A: the graphs represent G6PD activity (A,i) and relaxation (A,ii) of BCAs precontracted with KCl (30 mM) and treated with epiandrosterone (EPI) in a dose-dependent manner (n=10 experiments in each group). B,i: Western blot analyses were performed to demonstrate the expression of G6PD in the vascular tissue of G6PD-deficient (G6PD^Def) mice using polyclonal anti-G6PD antibody (B,i; top). β-Actin (ACTB) was used as a loading control (B,i; bottom). B,ii: force generation to KCl (30 mmol/L; n = 5 experiments in each group) by the coronary artery of G6PD^Def mice was measured as compared to wild-type (WT) mice.