Fig. 1.

Transient receptor potential family member (TrpC6) is expressed in the cytoplasm of dissociated cerebral vascular smooth muscle cells (VSMCs). VSMCs were enzymatically dissociated from a mixed pool of surface cerebral vessels, and their branches from a wild-type and TrpC6^−/− animal were then labeled with mouse anti-α-smooth muscle (SM) actin (green; far left) and rabbit anti-TrpC6 (red; middle). The merged image is shown at right. A faint signal is present in the TrpC6^−/− VSMCs; however, a greater signal, mostly cytoplasmic, is observed in the TrpC6^+/+ VSMCs.