Table 4. Nucleotide differences among the 23 cpDNA haplotypes detected in wild Cyclopia intermedia populations.
The three cpDNA fragments that were individually screened via High Resolution Melt analysis are presented as separate sets of columns, with the nucleotide position within the context of the concatenated haplotype provided for each base pair variation provided. The indels that differentiate haplotypes are reported below the table, with the variation among indel 2a–2e indicated by bold italicised typeface.
| MLT S3 –MLT S4 (atpI-atpH intergenic spacer) | MLT S1 –MLT S2 (atpI-atpH intergenic spacer) | MLT U1 –MLT U2(ndhA intron) | |||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Position | 23-29 | 30 | 35 | 38 | 66 | 91 | 99 | 116 | 130 | 136 | 147 | 191 | 192 | 197 | 233 | 272 | 304 | 306 | 321 | 338 | 342–448 | 437 | 442–448 | 462 | 498 | 538 | 569 | 587 | 613 | 677 | 712 | 726 | 739 | 794 | 797–801 | ||
| Consensus | 1 | T | T | T | A | C | C | G | A | T | T | C | G | A | G | C | A | C | G | G | 2a | G | 3 | T | G | C | G | T | T | G | C | G | G | C | – | ||
| Haplotype | |||||||||||||||||||||||||||||||||||||
| A | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | a | . | . | 2b | . | 3 | . | . | . | . | . | . | . | . | . | . | . | – | ||
| B | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | t | . | . | . | . | 2d | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4b | ||
| C | . | . | . | . | . | a | . | . | . | . | . | . | . | . | a | . | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | – | ||
| D | . | . | . | . | . | a | . | . | . | . | . | . | . | . | a | . | . | . | . | . | 2a | . | 3 | c | . | . | . | . | . | . | . | . | . | . | – | ||
| E | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | a | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | – | ||
| F | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4a | ||
| G | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | 3 | . | a | . | . | . | . | t | . | . | . | . | – | ||
| H | . | . | . | . | . | . | . | a | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | 3 | . | a | . | . | . | . | t | . | . | . | . | – | ||
| I | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | – | . | . | . | . | . | . | . | . | . | . | . | 4a | ||
| J | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | t | . | t | . | . | – | ||
| K | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | 3 | . | a | . | . | . | . | t | . | t | . | . | – | ||
| L | . | . | . | . | . | . | . | . | . | . | . | . | . | g | . | . | . | . | . | . | 2a | . | 3 | . | a | . | . | . | . | t | . | . | . | . | – | ||
| M | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | – | ||
| N | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | a | . | – | ||
| O | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | c | . | 2c | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4a | ||
| P | . | g | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | – | ||
| Q | – | . | a | a | . | . | . | . | . | . | . | t | . | . | . | . | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | t | . | . | . | . | – | ||
| R | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | t | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | – | ||
| S | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | t | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4a | ||
| T | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | t | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4b | ||
| U | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | t | . | . | . | . | 2e | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4a | ||
| V | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2e | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4a | ||
| W | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | – | . | 3 | . | . | . | . | . | . | . | a | . | . | a | 4a | ||
| C. aurescens | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | g | . | . | . | 2a | 3 | . | . | . | . | . | . | . | . | . | . | . | – | |||
| C. buxifolia | . | . | . | . | c | . | t | . | t | c | g | . | t | . | . | . | . | . | . | a | 2a | a | 3 | . | . | . | . | c | g | t | . | . | . | . | – | ||
| C. genistoides | . | . | . | . | c | . | . | . | t | c | g | . | t | . | . | . | . | . | . | a | 2a | a | 3 | . | . | t | a | c | . | . | . | . | . | . | – | ||
| C. longifolia | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | g | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4a | ||
| C. sessiliflora | . | . | . | . | . | a | . | . | . | . | . | . | . | g | . | . | . | . | . | . | 2a | . | 3 | . | . | . | . | . | . | . | . | . | . | . | 4b | ||
Notes.
1 = TAAAAAT, 3 = TATCTAA, 4a = TT—, 4b = TTTTC,2a = TACAGATGAAACGGAAGGGCTTCGTTTTTTGAATCCCTATCTAAATTTACAGTAACAGGGCAAA, 2b = TACAGATGAAAGGGAAGGGCTTCGTTTTTTGAATCCCTATCTAAATTTAAAGTAACAGGGCAAA, 2c = TACAGATGAAACGGAAGGGCTTCGTTTTTTGAATCCCTATCTAAATTTATAGTAACAGGGCAAA, 2d = TACAGATGAAACGGAAGGGCTTCGTTTTTTGAAAACCTATCTAAATTTACAGTAACAGGGCAAA, 2e = TACAGATTAAACGGAAGGGCTTCGTTTTTTGAATCCCTATCTAAATTTACAGTAACAGGGCAAA,