Fig. 2.

EryD-specific gene regulation is distal enhancer-driven. (A) Comparisons of EryP-/EryD-specific H3K27ac ChIP-seq peaks and their genomic distribution. (B) Categorization of distal accessible regions with H3K27ac. Heatmaps of ATAC-seq and H3K27ac ChIP-seq signal, centered at the ATAC-seq peak summits. Chromatin distal accessible regions were grouped into three groups: EryP-specific, EryD-specific, and shared peaks. Pie charts show the percentage of “active” (ATAC + H3K27ac) and “open” (ATAC-only) of each group, based on the presence of H3K27ac peaks. (C and D) Venn diagrams of EryP-/EryD-specific active enhancers (C) and super-enhancers (D). (E) Association study of EryP-/EryD-specific active enhancers and gene expression using C-score analysis. Rows are EryP-/EryD-specific genes. Columns are EryP-/EryD-specific enhancers. Also see SI Appendix, Fig. S2A and Table S3 and Materials and Methods.