Fig. 5.

Simulated predictions for tethered catalysis. (A) Simulations of k_cat from k_tet,max and k₃ based on Eq. 4 in a product release limited reaction. At high k_tet,max values, k_cat is decoupled from the rate of the tethered reaction and may disguise large differences in the tethered phosphorylation rate. (B) Fitting curves from Figs. 2C (WT) and 2E (R-3K) show unequal increase in k_tet (2.1× and 9.0×, respectively) upon increasing C_eff from 100 to 1,000 µM.