Fig. 2.

BMAL1 regulates c-Myc at the transcriptional level. (A) Expression of genes that regulate c-MYC degradation was measured by reverse transcription-qPCR using spleens of WT and Bmal1 KO mice and using gapdh as an internal control. Samples were collected at the indicated time points. Error bars correspond to SEM. Data were normalized to a value of 1 for WT at ZT0. (B) c-Myc mRNA levels in spleen samples detected by reverse transcription-qPCR. Three biological repeats were used for quantification. White and black bars indicate lights on and lights off, respectively. Error bars correspond to SEM. Data were normalized to a value of 1 for WT at ZT0. N.S, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, as determined by t test.