Fig. 3.

Cry1/2 double knockout decreases c-MYC expression in vivo. (A) Endogenous proteins c-MYC and β-catenin were detected by WB (Western blotting) in spleens of WT and three Cry mutants using GAPDH and Ponceau S as loading controls. Samples were collected at the indicated time points. (B–D) Quantification of c-MYC and β-catenin protein levels in the indicated genotypes. For each genotype and time point, three mice were used for quantification. White and black bars indicate lights on and lights off, respectively. Error bars correspond to SEM. For c-MYC, data were normalized to a value of 1 for WT at ZT8; for β-catenin, data were normalized to a value of 1 for WT at ZT4. N.S, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, as determined by t test.