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. 2020 Aug 19;117(35):21441–21449. doi: 10.1073/pnas.2000516117

Fig. 4.

Fig. 4.

FTO regulates VHL-deficient ccRCC cell growth and survival in an HIF-independent manner. (A and B) Western blot analysis of FTO, HIF1, and HIF2 expression in UMRC2-vec (A) and 786-OM1A-vec (B) cells transfected with indicated shRNA and siRNA. Actin was used as loading control. (C–F) Picture (Left) and quantification (Right) of 2D colonies (C and D) and 3D soft agar colonies (E and F) in UMRC2-vec (C and E) and 786-OM1A-vec (D and F) cells transfected with indicated shRNA and siRNA. (G) Western blot analysis of FTO and ARNT expression in shCtrl and shARNT UMRC2 cells transfected with siControl and siFTO. Actin was used as loading control. (H) Picture (Left) and quantification (Right) of 2D colonies in shCtrl and shARNT UMRC2 cells transfected with siControl and siFTO. Data represent the average ± SD. Student t test against corresponding control conditions (P < 0.05 and **P < 0.01).