Fig. 4.

Overexpression of G₁₂ inhibits arrestin recruitment to V₂R and receptor internalization. (A and B) Time course and concentration-dependence of BRET between V₂R-Rluc8 and β-arrestin2–Venus in response to AVP (1 μM in A; mean ± SEM; n = 3). Overexpression of G₁₂ but not G_s heterotrimers inhibits arrestin recruitment. (C and D) AVP-induced changes in BRET between V₂R-RlucII and the plasma membrane marker rGFP-CAAX (C) and the endosome marker rGFP-FYVE (D), indicating trafficking of V₂R-RlucII from the plasma membrane to endosomes (mean ± SEM; n = 6–8). Overexpression of G₁₂ but not G_s heterotrimers inhibits AVP-induced internalization of V₂R-RlucII.