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. 2020 Aug 19;117(35):21557–21567. doi: 10.1073/pnas.2000653117

Fig. 6.

Fig. 6.

The p40 inhibits the phosphorylation of STAT4 in MBP-primed T cells. Splenocytes isolated from MBP-immunized donor mice were restimulated with MBP (A–C) in the presence of IL-12 (D–F) and p40+IL-12 (G–I) for different time periods, followed by Western blot of STAT4 and pSTAT4 in nonadherent splenocytes. Cells were treated with p40 30 min before IL-12. Actin was used as a loading control. Bands were scanned, and values of pSTAT4/STAT4 (B, MBP; E, MBP+IL-12; H, MBP+IL-12+p40) and STAT4/β-actin (C, MBP; F, MBP+IL-12; I, MBP+IL-12+p40) are presented as relative to control. Data are expressed as the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.