Fig. 2.

Experimental γ-H2AX profiles by Mec1 and Tel1 when 50% of H2A sites cannot be phosphorylated. Phosphorylation spreading in strains where one of the two HTA genes is mutated to HTA-S129A, rendering half the H2A sites nonphosphorylatable. Experimentally measured γ-H2AX profiles by (A) Mec1 or (B) Tel1 are shown for 0 min (dashed lines) and 75 min (solid lines); “1/2 HTA1” refers to the presence of an hta2-S129A mutation, while “1/2 HTA2” carries the hta1-S129A mutation. Error bars represent SEM from n ≥ 3 measurements.