Fig. 5.

Fewer RGCs in adult SE mutants. (A) P30 retinal flatmounts immunostained with Rbpms antisera. SE/KO retinas have noticeably fewer Rbpms⁺ RGCs while KO/KO retinas have essentially none. Insets show 4×-magnified views. (B) Automated cell count data showing fewer total Rbpms⁺ RGCs in SE/SE, SE/KO, and KO/KO retinas than in +/+ retinas. (C) Plot showing how adult RGC counts vary with size of the RGC-competent progenitor pool, indicated by the planimetric density of E14.5 Atoh7⁺ cells. The curve rises steeply but flattens sharply for Atoh7⁺ cell densities >30% of WT. ***P < 0.001 and ****P < 0.0001. (Scale bar: 500 µm.)