Fig. 1.

Experimental set-up. (A) Schematic of selected metabolic reactions (BCAA - branched chained amino acids, AAA - aromatic amino acids, THF - tetrahydrofolate, TCA - tricarboxylic acid), in S. cerevisiae, involving the 14 supplemented amino acids (Asp, Arg, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Tyr, Val). Affected transporters during cultivations in rich medium are highlighted. (B) S. cerevisiae was cultivated in batch-phase, in well-controlled bioreactor conditions, and quantitative proteomics were analyzed for cells grown in aerobic and anaerobic conditions, with our without amino acid supplementation. (C) The allocation of 3,690 proteins was quantified for each of the 4 conditions, with a substantial overlap between all conditions. (D) PCA (principal component analysis)-plot highlighting similarities between individual replicates for examined conditions, at the proteomic level.