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. 2020 Aug 19;117(35):21391–21402. doi: 10.1073/pnas.2006997117

Fig. 2.

Fig. 2.

GABARAP can directly bind to STX17 and relieve the autoinhibited conformation of STX17. (A and B) Analytical gel filtration chromatography analyses of the interactions between GABARAP and STX17(142–228) (A) or STX17(1–228) (B). (C and D) ITC-based measurements of the binding affinities of GABARAP with STX17(142–228) (C) or STX17(1–228) (D). (E) Superposition plot of the 1H-15N HSQC spectra of STX17(1–123) (red), STX17(1–123) titrated with STX17(142–228) at a molar ratio of 1:1 (green), and STX17(1–123) saturated with STX17(142–228) followed by adding GABARAP at a molar ratio of 1:1:1 (blue). For clarity, the Insets show enlarged views of two selected regions of the overlaid 1H-15N HSQC spectra. DP, differential power measured by the ITC machine.