Fig. 1.

Schematic representation of the rpoN operon of P. aeruginosa PAO1, showing the position of the predicted P_hpf promoter sequence (located within the rpoN gene). Shown are the positions of the three transcriptional start sites (TSS1, TSS2 and TSS3) detected by RNA-Seq analysis of P. aeruginosa PA14 (Dotsch et al., 2012; Gill et al., 2018). The asterisk indicates that the TSS2 sequence was detected here using 5ʹ-RACE. Schematic representation of two of the single-copy hpf-yfp reporter constructs used to assay the relative promoter strengths of the P_rpoN and P_hpf promoters (all reporter constructs are shown in Fig S2).