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. Author manuscript; available in PMC: 2020 Sep 6.
Published in final edited form as: Mol Microbiol. 2018 Oct 15;110(2):161–175. doi: 10.1111/mmi.14001

Fig. 2.

Fig. 2.

A. hpf expression from PrpoN and Phpf promoters. Fluorescence (large symbols) and optical density (small symbols) of P. aeruginosa PAO1 containing single-copy Tn7 translational hfp-yfp fusions, during 48 h of growth. The control strains are wild-type P. aeruginosa PAO1 lacking a reporter construct (open rectangles), and PAO1 with an hpf-yfp translational fusion, but lacking a promoter sequence (closed rectangles). The Phpfhpf-yfp reporter (closed blue circles) contains the putative Phpf promoter sequence and UP element. The reporter containing both the PrpoN and putative Phpf promoters (open blue circles) had greater expression than the reporter containing Phpf alone.

B. hpf expression from PrpoN promoter, but lacking the Phpf promoter. The reporter constructs contained the PrpoN promoter and either a deleted rpoN gene (closed red circles) or a truncated rpoN gene (open red triangles).

C. RT-qPCR analysis of hpf expression due to transcriptional read through from PrpoN versus expression from Phpf. Results show the average of three biological replicates. Error bars show standard deviation.