Plasmids
pCAGEN-GFP (Addgene #11150)
pCAGN1- hCas9 (Addgene #51142)
pCAGN1- EV
pCAGN1-NOTCH2NL
pCAGN1-NOTCH2NL-T197I
pCAGN1-NOTCH2NL-M40I, T197I
pCAGN1-NOTCH2NL-N232S, T197I
pCAGN1-NOTCH2NL-E258A, T197I
pCAGN1-NOTCH2NL-M1I
pCAGN1-NOTCH2NL-M1I, T197I
pCAGN1-NOTCH2NL-M1I, M40I
pCAGN1-NOTCH2NL-M1I, N232S
pCAGN1-NOTCH2NL-M1I, E258A
pCAGN1-NOTCH2NL-HA
pCAGN1-NOTCH2NL-HA-T197I
pCAGN1-NOTCH2NL-HA-M40I, T197I
pCAGN1-NOTCH2NL-HA-N232S, T197I
pCAGN1-NOTCH2NL-HA-E258A, T197I
pCAGN1-NOTCH2NL-HA-M1I
pCAGN1-NOTCH2NL-HA-M1I, T197I
pCAGN1-NOTCH2NL-HA-M1I, M40I
pCAGN1-NOTCH2NL-HA-M1I, N232S
pCAGN1-NOTCH2NL-HA-M1I, E258A
pCAGN1-NOTCH2NL-HA-5′ M1
pCAGN1-NOTCH2NL-HA-5′ M1 + kozak
pCAGN1-NOTCH2NL-HA-5′ M40
pCAGN1-NOTCH2NL-HA-5′ M40 + kozak
pCAGN1-NOTCH2NL-HA-5′ M84
pCAGN1-NOTCH2NL-HA-5′ M84 + kozak
pCAGN1-NOTCH2NL-HA-5′ M1I-I1
pCAGN1-NOTCH2NL-HA-5′ P2
pCAGN1-NOTCH2NL-HA-5′ L12
pCAGN1-NOTCH2NL-HA-5′ P22
pCAGN1-NOTCH2NL-HA-5′ C28
pCAGN1-NOTCH2NL-HA-M1I-ΔI1
pCAGN1-NOTCH2NL-HA-M1I-ΔL4
pCAGN1-NOTCH2NL-HA, 5′ M1, CTG(1-5) > CTA(1-5)
pCAGN1-NOTCH2NL-M1I-HA, 5′ I1, CTG(1-5) > CTA(1-5)
pCAGN1-NOTCH2NL-M1I-HA, 5′ I1, Δata-CTG(1-5) > CTA(1-5)
pcDNA5-NOTCH2-GAL4-TAD-N1
pRL-CMV (Promega E2261)