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. 2020 Sep 6;6:81. doi: 10.1038/s41420-020-00315-w

Fig. 2. HNK augments autophagy in breast cancer cells.

Fig. 2

a Schematic diagram of the tfLC3 plasmid (upper panel). MDA-MB-231 and MCF7 cells were transfected with tfLC3 followed by treatment with 5 µM HNK or 200 nM rapamycin or Earle’s balanced salt solution (EBSS) for 24 h. Rapamycin and EBSS were used as positive controls for autophagic induction. Representative fluorescent images are shown. Scale bar: 10 µm. b MDA-MB-231 and MCF7 cells were treated with 5 µM HNK for 24 h followed by acridine orange staining. Representative images of MDA-MB-231 and MCF7 cells are shown. Scale bar: 15 µm. c, d Bar graphs show number of red and yellow puncta per cell in MDA-MB-231 and MCF7 cells transfected with tfLC3 and treated with 5 µM HNK or 200 nM rapamycin or EBSS for 24 h. ***P < 0.0005, compared with control.