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. 2020 Aug 28;36(9):1093–1094. doi: 10.1007/s12264-020-00564-w

Fig. 6.

Fig. 6

Activation of the ACC-to-MDL projection decreases vicarious freezing behavior. A Diagram showing optogenetic manipulation. B Tracing of projection from the ACC to the MDL. Left panel, AAV-hSyn-EGFP virus injection target in the ACC. Cg1, 2, cingulate cortex 1 and 2; right panel, projection terminals and optical fiber position in the MDL. C Left panel, AAV-hSyn-hChR2-EGFP virus injection target in the ACC; right panel, projection terminals and optical fiber position in the MDL. Nuclei stained with DAPI. D Light pulse-induced response of ChR2-expressing (upper and middle, 10 mW, 1 Hz and 5 Hz, respectively) and EGFP-expressing (lower, 10 mW, 1 Hz) ACC neurons in brain slice. E, F Freezing (E) and huddling (F) times of OSs in AAV-hSyn-EGFP (blue) or AAV-hSyn-ChR2 (red) groups. G Percentage freezing time of OSs in AAV-hSyn-EGFP- and AAV-hSyn-ChR2-injected groups in the retrieval period after fear conditioning. H Locomotor velocity of OSs in AAV-hSyn-EGFP (blue) and AAV-hSyn-ChR2 (red) groups, before and after light stimulation. Data are presented as the mean ± SEM; **P < 0.01, non-parametric rank-sum test, n.s., not significant.