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. 2020 Sep 7;9:16. doi: 10.1186/s13619-020-00055-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — The assessment of other types of cell death potentially involved in erastin-induced ferroptosis in ICCs. The protein expression changes of the protein markers for apoptosis and autophagy were examined after ICCs were challenged by 20 μM erastin for 24 h. Western blot results with the blot images (a) and quantification (b) on the protein expression of BAX, Bcl-2, P62, PARP, cleaved PARP (C-PARP) and LC3 I/II as well as actin were performed and analyzed. (n = 3 per group; *p < 0.05, **p < 0.01, ***p < 0.001. All data are expressed as means ± SEM)