Table 1.
Primer list.
| Gene | Forward primer: 5′-3′ | Reverse primer: 5′-3′ |
|---|---|---|
| NLRP3 | ATTACCCGCCCGAGAAAGG | TCGCAGCAAAGATCCACACAG |
| Caspase-1 | AATACAACCACTCGTACACGTC | AGCTCCAACCCTCGGAGAAA |
| IL-1β | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
| β-actin | GTGCTATGTTGCTCTAGACTTCG | ATGCCACAGGATTCCATACC |
| M1 markers | ||
| CD16 | TTTGGACACCCAGATGTTTCAG | GTCTTCCTTGAGCACCTGGATC |
| INOS | CAAGCACCTTGGAAGAGGAG | AAGGCCAAACACAGCATACC |
| TNF-α | CGTCGTAGCAAACCACCAAG | GAGATAGCAAATCGGCTGACG |
| M2 markers | ||
| Ym1/2 | CAGGGTAATGAGTGGGTTGG | CACGGCACCTCCTAAATTGT |
| IL-10 | TGGACAACATACTGCTAACCGAC | CCACTGCCTTGCTCTTATTTTC |
| TGF-β | TGCGCTTGCAGAGATTAAAA | CGTCAAAAGACAGCCACTCA |
NLRP3, NLR pyrin domain containing 3; IL, interleukin; INOS, inducible nitric oxide synthase; TNF-α, tumor necrosis factor alpha; TGF-β, transforming growth factor beta.