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Electrophoretic mobility shift assay (EMSA) of EjERF39 binding to the DRE element in the promoter of Ej4CL1 in vitro. (A) DRE elements in the Ej4CL1 promoter and oligonucleotides used for the EMSA, with the core sequences and base mutant indicated with lower-case letters. (B, C) Biotin-labeled DNA probe from the wild-type promoter or mutant probe was incubated with pGEX-EjERF39 protein. - and + represent the absence or presence of the indicated constructs.
