Fig. 2.

MdSOS2L1 interacted with MdALMT14. (A) BiFC was conducted in Nicotiana benthamiana to test the interaction between MdSOS2L1 and MdALMT14, with a plasma membrane marker (AtCBL1–red fluorescent protein (RFP)) included. (Scale bars: 100 µm.) (B) In vitro GST pull-down assays. MdSOS2L1–His proteins were incubated with immobilized MdALMT14–GST or GST, and the proteins immunoprecipitated with GST-beads were detected using anti-His antibody. (C) In vivo Co-IP assays between MdSOS2L1 and MdALMT14. Proteins were extracted from 35S::MdALMT14-Myc+35S::MdSOS2L1-GFP or 35S::MdALMT14-Myc+35S::GFP co-expressed in apple calli and immunoprecipitated with an anti-GFP antibody. The proteins from crude lysates (input) and the immunoprecipitated proteins (output) were detected with an anti-Myc antibody and anti-GFP antibody.