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. 2020 Mar 9;71(12):3437–3449. doi: 10.1093/jxb/eraa121

Fig. 4.

Fig. 4.

Cd2+ induced phosphorylation of the MdALMT14 protein. (A) Gel-shift assay of the MdALMT14 protein in the transgenic apple MdALMT14-OVX1 line using an anti-Myc antibody. Plants were treated with Cd2+ or with CIP for 1 h. (B) Western blot analysis detecting phosphorylation of the MALMT14 protein in the WT and transgenic MdALMT14-OVX1 plants using a specific anti-MdALMT14S358 antibody. (C) Cd2+-induced phosphorylation of the MdALMT14 protein increased with treatment time increased. WT ‘Gala’ apple plants were treated for different times (0, 1, 3, or 5 h). (D) Cd2+-induced phosphorylation of the MdALMT14 protein was absent in MdALMT14S358A–Myc transgenic apple calli using the anti-MdALMT14S358 antibody. For western blot assays, actin was used as a loading control to ensure equal loading.