Fig. 5.

MdSOS2L1 was required for Cd²⁺-induced phosphorylation of the MdALMT14 protein. (A) MdALMT14 phosphorylation was examined in 35S::MdALMT14-Myc transgenic apple calli, which were infected or not with the virus-based vector MdSOS2L1-TRV after Cd²⁺ treatment for 1 h. TRV empty vector-infected apple calli were used as the control. Western blot analyses were performed with an anti-MdALMT14^S358 antibody. (B) MdALMT14 phosphorylation in Cd²⁺-treated MdSOS2L1–GFP/MdALMT14–Myc and MdSOS2L1–GFP/MdALMT14^S358A–Myc calli, using an anti-MdALMT14^S358 antibody. (C) Western blot analysis using the anti-MdALMT14^S358 antibody showed the MdALMT14 phosphorylation in two overexpression lines, MdSOS2L1-OVX1 and -2, two suppression lines, MdSOS2L1-SUP1 and -2, and WT control with or without Cd²⁺ treatment.