Fig. 6.

MdSOS2L1-mediated phosphorylation stabilized the MdALMT14 protein. (A) Cell-free degradation assay of recombinant MdALMT14–GST or MdALMT14^S358A–GST protein in the protein extracts of transgenic apple calli, as labeled. Actin abundance was used as a loading control to ensure equal loading. Protein levels of MdALMT14–GST and MdALMT14^S358A–GST were visualized by immunoblotting with an anti-GST antibody. (B) MdALMT14 abundance in MdALMT14–Myc+GFP and MdALMT14–Myc+MdSOS2L1–GFP transgenic calli treated with Cd²⁺ stress.