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. 2020 Mar 9;71(12):3437–3449. doi: 10.1093/jxb/eraa121

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 7. — MdSOS2L1 increased the excretion of malate and Cd²⁺ tolerance in an MdALMT14-dependent manner. (A) Expression levels of MdALMT14 in MdSOS2L1-OVX1, MdSOS2L1-OVX1^shoot/(MdSOS2L1-OVX1+anti-MdALMT14)^root, MdSOS2L1-OVX2, and MdSOS2L1-OVX2^shoot/(MdSOS2L1-OVX2+anti-MdALMT14)^root transgenic plants. (B) The excretion of malic acid from the roots of MdSOS2L1-OVX1, MdSOS2L1-OVX1^shoot/(MdSOS2L1-OVX1+anti-MdALMT14)^root, MdSOS2L1-OVX2, and MdSOS2L1-OVX2^shoot/(MdSOS2L1-OVX2+anti-MdALMT14)^root transgenic plants grown under Cd²⁺ stress. (C) Cd tolerance of MdSOS2L1-OVX1, MdSOS2L1-OVX1^shoot/(MdSOS2L1-OVX1+anti-MdALMT14)^root, MdSOS2L1-OVX2, and MdSOS2L1-OVX2^shoot/(MdSOS2L1-OVX2+anti-MdALMT14)^root transgenic plants grown under Cd²⁺ stress. (D) Cd²⁺ content in the roots of MdSOS2L1-OVX1, MdSOS2L1-OVX1^shoot/(MdSOS2L1-OVX1+anti-MdALMT14)^root, MdSOS2L1-OVX2, and MdSOS2L1-OVX2^shoot/(MdSOS2L1-OVX2+anti-MdALMT14)^root transgenic plants grown under Cd²⁺ stress. (E) Leadmium^TM Green AM Dye showing Cd contents in the roots of MdSOS2L1-OVX1, MdSOS2L1-OVX1^shoot/(MdSOS2L1-OVX1+anti-MdALMT14)^root, MdSOS2L1-OVX2, and MdSOS2L1-OVX2^shoot/(MdSOS2L1-OVX2+anti-MdALMT14)^root transgenic plants grown under Cd²⁺ stress. The values are presented as the mean of three replicates, and differences with a P-value<0.05 were considered significant. Error bars represent SD. Significance was assessed with a t-test: *P<0.01.