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. 2020 Mar 28;71(14):4057–4068. doi: 10.1093/jxb/eraa161

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Time-course expression levels of RcCO, RcCOL4, and RcFT in TRV-RcCO and TRV-RcCOL4 plants under LD and SD conditions. (A–C) Relative expression of RcCO (A), RcCOL4 (B), and RcFT (C) in TRV-silenced Rosa chinensis ‘Old Blush’ under LDs. Error bars indicate ± the standard deviation (n=3). (D–F) Relative expression of RcCO (D), RcCOL4 (E), and RcFT (F) in TRV-silenced R. chinensis ‘Old Blush’ under SDs. Error bars indicate ± the standard deviation (n=3). The uppermost young leaves from 40-day-old plants propagated from cuttings were harvested and the total RNAs were extracted. RT-qPCR was then performed with RcGAPDH as reference gene. Every experiment was conducted with three replicates each with three technical repeats. The bar below the graphs indicates the light conditions, with day and night denoted in white and black, respectively.