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. 2020 Mar 28;71(14):4057–4068. doi: 10.1093/jxb/eraa161

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© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Transient transformation analysis of transcriptional activation of RcFT by RcCO and RcCOL4. (A) Schematic diagram of the reporter and effectors used in the Rosa chinensis ‘Old Blush’ transient transformation assays. (B and C) Representative images of transient expression assays in R. chinensis ‘Old Blush’ displayed by bright field (B) and dark field (C) of rose shoots expressing pRcFT-LUC together with 35S:empty, 35S:RcCO, 35S:RcCOL4, TRV-CO, TRV-COL4, TRV-CO+35S:RcCOL4, TRV-COL4+ 35S:RcCO, mutated RcCOL4-M1, and RcCOL4-M2. (D) Intensities of the LUC bioluminescence presented in (C) using Andor Solis image analysis software. Data are means ± SE (n=10). Different letters above the columns denote significant differences at P<0.05.