Fig. 5.
The loss of GID1a reduces xylem vessel proliferation and hydraulic conductance. (A, B) Total xylem vessel area (A) and xylem vessel number (B) in the base of the leaf petioles of M82 and gid1a (leaf no. 4, top down). (C) Theoretical specific hydraulic conductance (Kts) of the xylem vessels in M82 and gid1a. For calculation of Kt, the Hagen–Poiseuille equation (Tyree and Ewers, 1991) was used. To calculate Kts, Kt was normalized to the supported leaf area (Hochberg et al., 2015). (D) Volumetric-flow rate was measured in detached stem segments, taken from 4-week-old M82 and gid1a plants to calculate the actual hydraulic conductance (K′). (E) Total xylem vessel area in M82 and gid1a stems. Data in (A– E) (taken from six different plants) are graphically presented as box and whisker plots. Lowercase letters represent significant differences between respective lines (Student’s t-test, P<0.05). (F) Representative stem (as in E) cross sections of M82 and gid1a stained with Wiesner stain. Scale bar: 100 μm.