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. 2020 Aug;8(15):934. doi: 10.21037/atm-19-4784

Table 2. The sequences (5'-3') of primers and probes for MDM2 5 htSNPs examined.

rs number Primers and probes
rs1690924 Forward primer: TGTAATGGAAAGCCATCAGTAT
Reverse primer: TCTCCTGTCCCAAGATCTTGC
Common probe:-P-GCTATAAAAGATAATAGCATTTGTA-FAM-
Discriminating probe G: TTTAAAGACATGTATTAATGAGAAAACG
Discriminating probe A: AAAGACATGTATTAATGAGAAAACA
rs1846402 Forward primer: TAAGTGGGAGAGACAGAGAAC
Reverse primer: CCAGGTTAAGAACTTCTGCAC
Common probe:-P-GCTCAATCTGTCACTGAAAATCATGTTT-FAM-
Discriminating probe T: TTTTTTTTCACACTGAAATTCTGCCTAAGGTT
Discriminating probe G: TTTTTCACACTGAAATTCTGCCTAAGGTG
rs2291857 Forward primer: CTACTCAGTAGATATGCTATCAG
Reverse primer: CAACATTTAGTATGAGGATGC
Common probe:-P-ACCTTTCAATAAACATTAAACATGT-HEX-
Discriminating probe A: CTAACACAAACCCTTATGCAATTTA
Discriminating probe C: TTTCTAACACAAACCCTTATGCAATTTC
rs3730581 Forward primer: AGAAAATAGTTGACAGAGAGAA
Reverse primer: GCATGTACGAGATTCTGGTCT
Common probe:-P-TAGTAGACGAGAAGGCTGTTGCCTGTTT-HEX-
Discriminating probe G: TTTTTTTAATAGTTGAGAACAGTTAGTAGACG
Discriminating probe A: TTTTAATAGTTGAGAACAGTTAGTAGACA
rs3730635 Forward primer: AAGGTGGAAGAGCCTTTTCAG
Reverse primer: CGAAAGTACCTACAGTGTGAC
Common probe:-P-GTTAGAGGGGGAAAGTGTGGAAGTT-FAM-
Discriminating probe G: TTTGGATTTTGAAACTGAAATTATTCTG
Discriminating probe A: GGATTTTGAAACTGAAATTATTCTA