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. 2020 Aug;8(15):929. doi: 10.21037/atm-20-1054

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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eUb upregulated the ratio of M2 macrophages in tumor-bearing mice. (A,B) The peripheral blood of tumor-bearing mice was collected, and the ratios of macrophages and Tregs were analyzed by flow cytometry. (C) The lung tissues of the tumor-bearing mice were harvested to detect the proportion of M2 macrophages via immunofluorescence assay, the arrows indicated M2 macrophages (×200). (D) The cytokines contained in the serum of tumor-bearing mice were measured by ELISA. (E) Lung tissues were collected to detect the proteins related to macrophage polarization through Western blot. GAPDH was used as a loading control. *, P<0.05. CON, control.