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eUb promoted M2 macrophage polarization in vitro. THP-1 monocytes were preincubated with PMA (100 nM) for 24 h to induce M0 macrophages. After M0 macrophages were treated with eUb (1, 2, 5, 10 µg/mL) for 72 h, the mRNA expressions of macrophage markers were measured by qPCR and normalized by GAPDH expression (A), the expressions of macrophage markers CD206 and CD86 were analyzed by flow cytometry (B). *, P<0.05. CON, control.
