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. 2020 Aug;8(15):950. doi: 10.21037/atm-20-2901

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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RACGAP1 is regulated by E2F3. (A) Transcription factor analysis showed that RACGAP1 co-expression genes are regulated by E2F family members. (B) QPCR assay confirmed the efficiency of siRNAs. (C) QPCR assay showed that Only E2F3 knockdown could repress the mRNA expression of RACGAP1 in KYSE140 and KYSE180. (D) QPCR assay showed that E2F3 overexpression induced the mRNA expression of RACGAP1. (E) WB assay confirmed that RACGAP1 is regulated by E2F3. (F,G) Luciferase reporter assay showed that E2F3 knockdown decreases the promoter activity of RACGAP1 (F), and E2F3 overexpression increase the promoter activity of RACGAP1 (G). (H,I) E2F3 mRNA level is positively correlated with the RACGAP1 mRNA level in ESCC cell line RNA-seq data (H) and TCGA ESCC data (I). (J) RACGAP1 mRNA level is significantly higher in E2F3 copy number gain ESCC samples than E2F3 copy number not-gain ESCC samples. The data are presented as means ± SEMs. The P values were determined with Student’s t-test (two groups) or one-way ANOVA (three groups). **, P<0.01; ***, P<0.001. ESCC, esophageal squamous cell carcinoma.