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. 2020 Aug;8(15):933. doi: 10.21037/atm-20-3871

Figure 3.

Figure 3

Treatment with valproic acid (VPA) as a histone deacetylase (HDAC) inhibitor improves the efficiency of the hypoxia/neuron-specific gene expression system. (A,B) Representative in vivo imaging system (IVIS) images and quantification of luciferase expression in induced neural stem cells (hiNSCs) transfected with NSE (A) or EpoNSE plasmids (B). Transfected hiNSCs were maintained under normoxia for 24 h after treatment with an HDAC inhibitor. (C) Representative IVIS image and quantification of luciferase expression in hiNSCs transfected with EpoNSE plasmid. Cells were maintained under hypoxia for 24 h after treatment with an HDAC inhibitor. (D) Comparison of luciferase expression in hiNSCs transfected with NSE or EpoNSE plasmids. Cells were maintained under hypoxia for 24 h after treatment with an HDAC inhibitor. (E) Representative IVIS image and quantification of luciferase expression in EpoNSE-hiNSCs transplanted into the injured spinal cord 24 h after transplantation. *P<0.05. ACL or ACLV, A, A83-01; C, CHIR99021; L, leukemia inhibitory factor; V, valproic acid.